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Abstract This work was carried out to study the zoonotic importance of Vibrio spp. as potential food-borne pathogens. To achieve this purpose, the following points were investigated: I- Occurrence of Vibrio spp. in the examined samples. • A total of 400 samples (280 shrimp and 120 tilapia) were collected from different fish markets in Sharkia Governorate. In addition, 200 humans’ stool samples were also collected from diarrheic (n=120) and non-diarrheic patients (n=80) referred to Zagazig hospitals, Sharkia Governorate. • The results revealed that 17% (102/600) of the samples were positive for Vibrio spp. based on bacteriological and molecular examinations. • The overall frequencies of different Vibrio spp.; V. parahaemolyticus, V. cholerae, V. vulnificus, V. alginolyticus, V. mimicus, V. fluvialis were 5.7, 0.7, 2.2, 4, 3.8 and 0.7%, respectively. II-Occurrence of Vibrio spp. in shrimp and tilapia fish • The overall isolation rate of Vibrio spp. was 24.8% (99/400). The percentage of infection was 28.2 and 16.7 in the examined shrimp and tilapia fish samples, respectively. • The frequency of V. parahaemolyticus, V. cholerae, V. vulnificus, V. alginolyticus, V. mimicus, V. fluvialis in the examined shrimp versus tilapia samples was (8.9 vs. 5%), (0.7 vs.1.7%), (3.2 vs. 3.3%), (7.1 vs. 3.3%), (6.8 vs. 3.3%) and (1.4 vs. 0), respectively. |