الفهرس 
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Abstract
Diabetes mellitus (DM) is associated with severe progressive degenerative complications in many organs mainly diabetic nephropathy (DN). Adipose-derived stromal vascular fraction (SVF) is easily obtained and has abundant viable stem cell number obviating extensive expansion in culture, thus SVF utilization provides promising anticipation. Low laser intensity irradiation (LILI) was found to strengthen the therapeutic effect of non-expanded SVF through enhancing viability, protein expression and migration of stem cells. The current study aimed to demonstrate the effect of laser activated SVF IP transplantation in streptozotocin (STZ) induced diabetic rats. Forty Sprague Dawley male rats were randomly and equally divided into four groups; (I) control group, (II) diabetic untreated group, (III) and (IV) diabetic treated groups in which laser activated SVF was transplanted as single and multiple IP injections, respectively. DM was induced by single IP injection of STZ at a dose of 55 mg/kg B.W. group III and IV received the SVF at a dose of 1.5x106 nucleated cells/ rat IP at the 7th day of DM induction, 2 weeks later group IV received the second SVF injection. Adipose tissue was collected from abdominal subcutaneous region, digested, centrifuged and SVF pellet was activated using low intensity laser irradiation. Clinical signs observation, insulin gene expression quantification, glycemic profile evaluation (blood glucose level, C-peptide and glycosylated hemoglobin), in addition to hematological, biochemical, histopathological, and microalbuminuria parameters were assessed in the different experimental groups. Both treated groups exhibited improvement in glycemic profile which confirmed by significant decrease in blood glucose level with increase of insulin gene expression and C-peptide level, moreover there was a significant improvement in leukogram, creatinine, potassium, and urea levels. Erythrogram, serum transaminases, lipid profile, sodium levels, and microalbuminuria were normalized in treated groups. Laser activated SVF transplantation in diabetic rats triggered improvement of the diabetic state and ameliorated some of its complications, mainly diabetic nephropathy state, regards to the early interference. SVF multiple IP injections strategy is recommended for treatment rather than single one.