الفهرس 
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Abstract
Escherichia coli (E. coli) is the most common bacteria associated with asymptomatic bacteriuria in patients with orthotopic ileal neobladdder. We aimed to characterize these isolates as regard virulence phenotype, phylogrouping and prevalence of pathogenicity islands (PAIs). METHODS: 35 E. coli isolates from monobacterial urine cultures collected from 35 patients with orthotopic ileal neobladder were compared to 35 uropathogenic E. coli (UPEC) isolates from 35 patients with native bladder suffering from UTIs and 30 fecal E. coli isolates from healthy adults. Isolates were compared as regard prevalence of phenotypic virulence traits (α-hemolysin production, biofilm formation and hemagglutination pattern), distribution of phylogenetic groups (A, B1, B2, D) and frequency of PAIs carriage. RESULTS: The neobladder isolates showed that the least resistance was to amikacin (0%) followed by meropenem (5.7%), gentamicin (11.4%), piperacillin-tazobactam (14.3%), and nitrofurantoin (22.9%). Neobladder isolates was found to be intermediary between both UPEC and the fecal isolates except for mannose sensitive hemagglutination that was more prevalent in neobladder isolates (28.6%) compared to UPEC (25.7%) and fecal (10%) isolates. Phylogrouping of neobladder isolates was: A (42.9%), D (37.1%), B2 (17.1%), and B1 (2.9%). As regard bladder isolates, group B1 was not detected, while group A and B2 were equally distributed, each represents (37.1%), followed by group D (25.8%). Among fecal isolates phylogroup A (50.0%) was the most prevalent followed by groups D (30.0%), B1 (13.3%), and B2 (6.7%). Significantly, phylogroup B1 was more prevalent in fecal isolates, while, group B2 was more common in bladder isolates. No significant difference present as regard the distribution of the phylogroups when comparing the neobladder isolates either with bladder or fecal ones. Among the 100 E. coli isolates, 77 isolates (77%) carried at least one PAI marker among which a total of 129 PAIs were detected including 25 fecal isolates (83.3%) carrying 36 PAIs, 29 bladder isolates (82.9%) carrying 49 PAIs, and 23 neobladder isolates (65.7%) carrying 44 PAIs (p > 0.05). The most prevalent PAIs were PAI IV536 (56%) and PAI IICFT073 (37%), respectively, while PAI IIJ96 was not detected in any of the isolatesThe maximum number of PAIs detected per isolate was 4 (II536, III536, IV536, IICFT073), found in one noebladder isolate of the phylogroup B2. CONCLUSIONS: While distinct, the distribution of phenotypic virulence traits among neobladder isolates were intermediary between both UPEC and fecal isolates, revealing its ability to colonize the ileal neobladder but rarely progress to symptomatic UTI. The predominant phylogenetic group among the neobladder isolates were A and D, the same as in fecal isolates. The prevalence of PAIs carriage among neobladder E. coli isolates was less than that detected among UPEC and fecal isolates.