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Abstract In Egypt, SPP and CPD diseases are one of the endemic skin diseases and its contagious nature affecting sheep and goat’s health due to highly mortality rate, particularly in lambs and kids, as well as their long-term effects on the livestock industry due to persistent scabs. Therefore, the aim of the study is to investigate the SPPV and Orf virus infection based on a characteristic signs of infected sheep and goats, along with PCR technique using a specific primer. Evaluation of a commercial RSPPV vaccine comparing with a candidate trivalent SGPV vaccine containing both SPPV (RSPPV, KSPPV) and GTPV strains with different assays. In addition to the relationship between a candidate SGPV vaccine and a local SPPV isolate. An outbreak of SPP disease during April 2017 on a non-vaccinated local breed of sheep in Kafr Shalshamoun, Menya Al Qamh, Sharkia, Egypt, include 85 sheep with morbidity rate was 23.5% and the mortality rate was 8.2%, respectively. The infected sheep were suffered from increase of body temperature, nasal discharge, lacrimation, and nodules formation on head, face, ears, nostril, inside the lips, inner aspect of thigh and ventral aspect of the tail. PCR based on ORF 103 gene-specific primers used to detect SPPV in collected scabs and nasal-oral swabs from clinically infected animals. A single band of expected product . |