![]() | Only 14 pages are availabe for public view |
Abstract The present study was carried out on 80 samples of fast served meat meals including (20 each of Kofta, liver, shawerma and hawawshi) were collected from different localities (fast food restaurants and streetvendors) with different sanitation levels in Zagazig City, Sharkia governorate, Egypt. All collected samples were examined to determine the prevalence of food poisoning microorganisms in fast served meals I. Organoleptic evaluation of fast served meat sandwiches: All examined sandwiches samples were normal in color (well-done brown), accepted fresh in odor, palatable in taste and firm and tender in consistency. II. Bacteriological examination: II.1. Isolation and identification of S. aureus: The recorded results showed that the prevalence of S. aureus was 20%, 15%, 20%, 25% in the examined kofta, liver, shawerma and hawawshi, respectively with a mean value of 4.21± 0.575, 4.83± 0.423, 4.79±0.475, 4.32±0.512 log 10 cfu/g, respectively. With minimum count 3, 3, 3, 2.5 log 10 cfu/ g and maximum count 7, 7.3, 7.3, 7.7 log 10 cfu/g. In general all examined samples were classified according to S. aureus count into good 64 (80%), acceptable 2 (2.5%), unsatisfactory 6 (7.5%) and potentially hazardous 8(10%). In this study the isolates of S. aureus were also tested for demonstration of enterotoxins and mecA virulence gene of MRSA (methicillin resistant Staphylococcus aureus) by multiplex PCR. The obtained data showed that only four .strains out of 16 isolated coagulase |