الفهرس 
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Abstract
Our research aim to study the effect of Epigallocatechin-3-gallate on colon cancer and to reveal that we measuring the following parameters:-.Cell viability by Methyl Thiazolyl Tetrazolium (MTT assay), molecular study by using Real Time Polymerase Chain Reaction for Basic fibroblast growth factor (BFGF), Vascular endothelial growth factor (VFGF) and Caspase-3 Using Real time polymerase chain reaction.
This experiment was done
The result showed that
Epigallocatechin-3-gallate has a concentration inhibitory effect on HCT- 116 cells and no effect on normal cells. Epigallocatechin-3-gallate inhibited the growth of HCT-116 cells in a concentration-dependent manner. At the concentration of 4.5μmol, 50% viability was detected during the 48 hr treatment, whereas maximum cytotoxicity LC90 was observed at a concentration of 8.1μmol
in vitro cytotoxic effect of Epigallocatechin-3-gallate on normal cells RPE1 (normal retina cell line) after 48 hr. treatment with different concentration of Epigallocatechin-3-gallate ranged between 0.78 to 100 µmol/ml. The result shows that had no inhibitory effect on the viability of RPE1 cells
VEGF gene expression was significantly decreased with 12.5 µmol 25 µmol and 50 µmol Epigallocatechin-3-gallate treatments while showing non- significant reduction with 100µmol of Epigallocatechin-3-gallate in HCT-116 cells.
BFGF gene expression was significantly decreased with 12.5 µmol Epigallocatechin-3-gallate treatment beside non-significant reduction with 25 µmol, 50 µmol and 100 µmol of Epigallocatechin-3-gallate in HCT -116 cells.
Caspase -3 gene expression was significantly elevated with increase concentration of Epigallocatechin-3-gallate treatment in HCT-116 cells.