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العنوان
Effect of some extracts from plant origin on musca domestica l. =
المؤلف
Ahmed, Noha Naguib.
هيئة الاعداد
باحث / Noha Naguib Ahmed
مشرف / Nashaat Labib Qulada
مشرف / Hossam El-Deen Magdi Zahran
مشرف / Hedayat Abdel Ghaffar Abdel Halim
الموضوع
Entomology, Economic.
تاريخ النشر
2018.
عدد الصفحات
84 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الزراعية والبيولوجية
الناشر
تاريخ الإجازة
12/12/2018
مكان الإجازة
اتحاد مكتبات الجامعات المصرية - الحشرات
الفهرس
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Abstract

The present study included two parts:
A- Evaluation of the biological effects of some essential oils on the developmental stages of M. domestica.
1. Susceptibility levels of M. domestica larvae following treatments with the tested essential oils were determined.
a- The results showed that the highest toxicity against house fly larvae was observed at concentration 0.033 mg/l (LC50 value) of brush bottle oil after 48 hrs. followed by Lantana oil at concentration 0.33 mg/l (LC50 value). While the camphor oil had the lowest toxicity was 2.05 mg/l (LC50 value) after 48 hrs.
b- The biological effects of essential oils showed that the brush bottle oil had the highest effect on pupation at concentration 0.03 mg/l gave 37% and at 1mg/l it´s gave 0% , the lowest effect on pupation was in camphor oil.
c- The results showed that the brush bottle oil had the most effective on adult’s emergence and it was 0% at 1 mg/l, while the adult emergence was 81% at concentration 0.3 mg/l of camphor oil.
d- The tested oils had a clearly effected on fecundity as compared with control, the highest effect was 0% at concentration 0.5 mg/l for brush bottle oil and the lowest effect on fecundity was in camphor oil.
e- All the tested oils were significantly reduced fertility values and the highest effect was observed on brush bottle oil 0% at 1 mg/l and the lowest was on camphor oil 94% at 0.3 mg/l
B- Effect of tested essential oils on the blood picture of last larval instar of M. domestica.
1. The efficiency of the tested essential oils on the haemolymph weight of M. domestica last larval instar.
The results showed that the larval treatments with LC25 of tested essential oils led to a reduction in the larval haemolymph weight by about 78.03 % in lantana oil, 14.73% in brush bottle oil, 13.68 % in thyme oil, in clove oil 21.47% , in camphor oil 5.83 % and in rosemary oil was 7.44% after 16 hrs of treatment with tested essential as compared with the control.
2. The efficiency of the tested essential oils on the different haemocyte counts on M. domesticalast larval instar.
The results indicated that treatment with LC25 of tested essential oils decreased the total haemocyte count in last larval instar by about 78.03% in lantana oil after 4hr of treatment, 33.3% in brush bottle oil, 24.4 % in thyme oil, in clove oil was 99.3 %, in camphor oil was 23.3 % when compared with the control. While in the rosemary oil, the THC was increased about 28.9% more than control.
3. The efficiency of the tested essential oils on the different haemocyte counts (DHC) on M. domesticalast larval instar.
The results indicated that larval treatments with LC25 of tested essential oils decreased the different haemocyte count except rosemary oil. Treatment last larval instar with lantana oil decreased the different haemocyte count by about 57.30% in prohaemocyte, 35.5% in
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granulocyte, 40% in plasmatocyte and 29.3 % in oenocytoides, respectively after 16hrs of treatment. The same trend was observed in brush bottle oil treatment by about 48.7 % in prohaemocytes7.5 % in granulocyte, 28.7% in plasmatocytes and 17.2 % in oenocytoides, respectively. In thyme oil treatment decreased the DHC by about 12.7% in prohaemocytes 4 % in granulocyte 7.14% in plasmatocytes and oenocytoides about 44% in, respectively. In clove oil treatment decreased the DHC by about 21.1% in prohaemocytes, 17.2 % in granulocyte, 10.4% in plasmatocytes and 29.3% in oenocytoides, respectively and in camphor oil treatment DHC decreased by about 40.4 % in prohaemocytes, 24.5 % in granulocyte, 20.3% in plasmatocytes and 17.7% in oenocytoides. While in the rosemary oil the DHC was increased about 9.6% prohaemocytes, 6.4% in granulocyte, 6.14% in plasmatocytes and 24.9% in oenocytoides more than control.
C. The efficiency of the tested essential oils on M. domestica larval genomic DNA.
The present results clearly show that treatment with lantana and clove oils caused abnormal increases in DNA fragmentations in different sites of the larval DNA, compared to untreated larvae .In contrast, treatment with brush bottle tree oil caused disappeared in some bands, this result suggests that treatment with tested essential oils were effective on DNA synthesis.
Finally, we conclude that the nature possesses numerous medicinal plants, which may be useful for control of many vectors borne diseases. The results of this study demonstrated that the lantana and brush bottle tree essential oils have excellent house fly larvicidal activity.
Finally, we conclude that the nature possesses numerous medicinal plants, which may be useful for control of many vectors borne diseases. The results of this study demonstrated that the lantana and brush bottle tree essential oils have excellent house flylarvicidal activity.