الفهرس 
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Abstract
Febrile neutropenia (FN) is the evolution of fever in a patient with neutropenia over 38.0 C. Neutropenia diagnosed when absolute neutrophil count (ANC) <1500 cells / micro L. FN represents a common complication of cancer treatment and hence a major cause of morbidity and mortality in cancer patients. Staphylococcus aureus is one of the most important microorganisms isolated from blood of febrile neutropenic patient. Infections caused by S. aureus range from mild to life-threatening diseases. Biofilm production by S. aureus is one of the most important virulence factors to the bacteria as it prevents penetration of antibiotics. It has recently been shown that S. aureus carries the ica operon responsible for biofilm production. In this study, relation between presence of icaA and icaD and biofilm production was determined in a collection of 66 S. aureus from febrile neutropenic patient. Biofilm -forming ability was tested on Congo Red agar plates; 48 were positive (biofilm -productive) and 18 were negative (non- biofilm productive). Biofilm forming S. aureus isolates were tested for icaA and icaD genes. 37.5% were positive to icaA, 22.9% were positive to icaD and 10.4% were positive to both. The aim of this work was to determine a genotypic characterization include detection of icaA and icaD genes in S. aureus, also to determine their relation to clinical and microbiological features. Empiric antibacterial was recommended for cancer patients receiving chemotherapy. Effect of the most sensitive antibiotics on bacterial cell was determined by electron microscope. We found that the most effective antibiotic was Linezolid. Our research recommends usage of Linezolid in treatment of febrile neutropenic patient caused by biofilm productive S. aureus. We found severe changes appeared on the bacterial cell after treated with Linezolid and existence of icaA and icaD genes was only in 50% of biofilm productive bacteria.