الفهرس 
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Abstract
The aim of the study was to evaluate the effect of EDTA and Phytic acid on attachment and differentiation of dental pulp stem cells (DPSCs). The null hypothesis is that different root canal irrigation protocols alter the survival of dental pulp stem cell (DPSC). Material and methods: Dental pulp stem cells were obtained from healthy impacted molars..characterization of stem cells was done and verified using mesenchymal stem cell surface antigen of cell surface CD marker by flow cytometric. The coronal dentin cut into a square shape and conditioned with different solutions as EDTA 17% and Phytic acid 1% and 5%.. Cell attachment was evaluated based on the gene expression level of fibronectin 1 which is the key adhesion protein. The proliferation and cytotoxixity of dental pulp stem cells (DPSCs) on the conditioned dentine specimens was analyzed using MTT assay and the cellular attachment and morphology were also observed by Scanning Electron Microscopic(SEM). To invitegate the differentioation capacity of dental pulp stem cells into odontoblast like cells we compare differential gene expressions under various conditions, qRT-PCR analyzed of the mineralization-related genes was performed and stained with Alizarin Red for detection matrix mineralization used an inverted phase-contrast microscope. Results : High purity of dental pulp stem cells showed by Flow cytometric analysis.. EDTA presented the highest degrees of enlargement in dentinal tubules and smear layer removal then IP6 5% and finally IP6 1%. None of the experimental groups showed cytotoxicity on DPSCs. IP6 5% group showed a higher percentage of viability compared with the other groups. There was no statistical significance between the different growth factors groups or the inductive media group regarding DPSCs odontoblast differenttion except for the TGFβ1 group, which showed the highest calcification. DPSC proliferation rate increased significantly in IP6 5% and EDTA 17 % with the time, whereas DW and IP6 1% groups showed the lowest proliferation rate. The calcification nodules were observed in the IP6 5% group and EDTA 17 % significantly and were more expanded and homogenously distributed in IP6 %5. Conclusion: The phytic acid 5% conditioning could be useful and may have beneficial effects on regenerative endodontic treatment and might be a potential candidate for dentin-pulp complex regeneration.