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Abstract This investigation was carried out during 2016 2017 seasons in privet vineyard located at EL-Sadat city, menoufiya governorate on Flame seedless grapevines seven years old the selected vines are planted at 2 x 3 meters apart. The soil texture is sandy. Pruning was conducted on the end of December in both seasons leaving 72 eyes per vine (16 fruiting spure x 4 eyes) + ( 4 renewal spurs x 2 eyes ). The main objective of this experiment was tostudy the effect of different organic manure sources namely compost, poultry, waste of cities and manure application method i.e. superficial and trench application, besides two biofertilizers (N- fixing bacteria) namely Nitrobin and Rhizobacterien as well as their interaction on vegetative growth, leaf mineral content, yield and its components as well as fruit quality attributes. This present experiment included the following treatments:- 1. Poultry manure with trench application + Rhizobacterien 2. Poultry manure with trench application + Nitrobien 3. Poultry manure with superficial application + Rhizobacterien 4. Poultry manure with superficial application + Nitrobien 5. Compost with trench application + Rhizobacterien 6. Compost with trench application + Nitrobien 7. Compost with superficial application + Rhizobacterien 8. Compost with superficial application + Nitrobien 9. City west compost with trench application+ Rhizobacterien 10. City west compost with trench application + Nitrobien 11. City west compost with superficial application+ Rhizobacterien 12. City west compost with superficial application + Nitrobien The results of the study could be summarized as follows: 1. Vegetative growth a. Shoot length Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant shoot length was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. b. Number of leaves Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant number of leaves was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. c. Leaf area Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant leaf area was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. 2. Leaf mineral content a. Nitrogen Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant leaf nitrogen content was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. b. Phosphorus Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant leaf phosphorus content was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. c. Potassium Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant leaf potassium content was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. 3. Yield and its components a. Yield/vine Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant yield/vine was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. b. Number of clusters Regarding different organic manure sources with application method treatments, it is obvious that no significant difference among them in both seasons of study. With respect to bio-fertilizers application, data show that no significant difference between Rhizobacterien and Nitrobien applications in both seasons of study. obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. b. Size of 100berry Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant size of 100berry was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. 5. Chemical characteristics of berries a. TSS Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant percentage as compared to with superficial application. Highest significant berry TSS juice was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest percentage as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant percentage was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. b. Acidity Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the least significant percentage as compared to with superficial application. Least significant berry acidity juice was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the least percentage as compared to Nitrobien application, which it had significantly the highest one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the least significant percentage was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the highest one in both seasons of study. c. TSS/acid ratio Regarding different organic manure sources with application method treatments, data clarify that all organic manure sources with trench application had the highest significant values as compared to with superficial application. Highest significant berry TSS/acid ratio juice was obtained with poultry manure followed by compost, while city west compost came in the third position in both seasons of study. With respect to bio-fertilizers application, data show that vines receiving of Rhizobacterien application produced significantly the highest values as compared to Nitrobien application, which it had significantly the least one in both seasons of study. Concerning interaction between type of organic manure with application method and bio-fertilizers application, the highest significant values was attained by poultry manure with trench application + Rhizobacterien, whereas city west compost with superficial application + Nitrobien resulted in significantly the least one in both seasons of study. from the foregoing results, it can be concluded that application of poultry manure with trench application + Rhizobacterien was the best management system for achieving the best vegetative growth parameters,leaf mineral content and ensuring the best yield with its components as well as fruit quality at tributes of Flame Seedless grapevines. |