الفهرس 
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Abstract
The aim of this study was to evaluate the protective effect of aqueous extract of Bael (Aegle marmelos) leaves against cisplatin (Cis) induced hepatotoxicity and nephrotoxicity in Rats. The chemical composition, polyphenolic compounds and 1,1-diphenyl- 2 -picryl hydrazyl (DPPH) radical-scavenging activity of Aegle marmelos aqueous extract (AMAE) were determined. Forty-two adult male Sprague-Dawley rats (average body weight was 200±10 g) were divided randomly into six groups as follow: group 1: negative control, was fed on basal diet and received appropriate volume of saline intragastrically daily. Groups 2 and 3 were received AMAE by dose 400 or 700 mg /kg body weight, p.o, respectively. group 4: positive control, was injected with Cis (7 mg / kg boy weight i.p). Groups 5 and 6 were treated with AMAE by dose 400 or 700 mg /kg, respectively, and injected with Cis. At the end of the experimental period (4 weeks), rats were scarified and serum was collected for biochemical analyses. Quantitive phytochemical analysis of AMAE have revealed the possession of active phytochemical constituents such as vanillic acid, benzoic acid, ellagic acid and salicylic acid with values 887.3918, 635.9879, 747.1384 and 773.3685 mg / kg, respectively. The antioxidant activity (DPPH) of A.MAE was recorded to be 95.02% for the high tested level (5% of smples). The administration of Cis resulted in significant elevation in serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Also serum urea and creatinine levels were significantly elevated whereas serum total proteins and albumin were significantly reduced. These effects were accompanied with increased levels of serum tumor necrosis factor-α (TNF- α) and interleukin-1 beta (IL-1β), along with significant elevation of hepatic and renal malondialdehyde level (MDA) and significant depletion in the level of reduced glutathione (GSH) in the same organs as compared with negative control. Treatment with AMAE prior to Cis produced protective effects and attenuated these biochemical changes. The protective effects of AMAE were more pronounced for the high dose. The histological examination confirmed the protective effects of AMAE pretreatment against Cis induced toxicity. Also, AMAE by the two tested doses did not show any negative physiological effects throughout the time of the study (4weeks) and this was point to its safety for human use. In conclusion, pretreatment with Aegle Marmelos extract led to a significant attenuation of the liver and kidney injuries induced by Cis. The protective effects of AMAE might be ascribable to its anti-inflammatory and antioxidant properties.