الفهرس | Only 14 pages are availabe for public view |
Abstract • Tomonitortheabilityofthefood-borneopportunisticpathogenB.cereustosurviveduringminimalprocessingoffoodproducts,this studywasdesignedtodeterminetheisolationrateofB.cereusgroupbacteriaindifferentdairyproductsandfastfoodssamples andtostandupontheirphysiologicalandmolecularadaptive responsesagainstheat,acidicpH,coldas wellasosmoticsalt stresses.Inaddition,theE.coligrowthretrievalupon supplementationwithB.cereusorganicextracellularfractionswas alsoassessed. • Atotalof200variousmilk,milkproductsandfastfoodsamples collectedfromdifferentsupermarketsandrestaurantswas subjectedfordeterminingtheexistenceofB.cereusgroupspecies byconventionalmicrobiologicaltechniques.Theeffectsofheat, cold,acid,andsaltonB.cereusheatresistancewas physiologically evaluatedthroughdetectionoftheirsurvivalandthermotolerance, whiletheyweremolecularlyevaluatedthroughmeasuringthegene expressionofB.cereusheatinducedproteins(mainlychaperones; GroELandDnaJ) byRT-qPCR assay.Finally,theassessmentofE.coligrowthretrievaluponsupplementationwithB.cereusorganic extracellularfractionscomparingwithinorganicsupplementswas phenotypicallyandgenotypicallydone • Ourdatashowedthat B.cereus-likebacteriawereisolatedfrom manycategoriesoffoodstuffs(dairyproductandfastfoods) samples,whileitisfrequentlypresentassporesinthe environments.Inonly7%themilkandfastfoodssamples,the countswere>104CFU/mLorg,whereasin4.5%ofthesamples, |