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العنوان
Reverse transcriptase -qPCR for assessment of Bacillus cereus survival after various stress treatments /
المؤلف
.Yonan, Engy YonanAyoub
هيئة الاعداد
باحث / أنجى يونان أيوب يونان
مشرف / حلام عبدالعزيز غريب
مشرف / مروة أبراهيم إبراهيم
مناقش / فتحى محمد السيد مصطفى
مناقش / اشرف محمود عمر
الموضوع
Bacillus cereus. Stress- veterinary.
تاريخ النشر
2021.
عدد الصفحات
134 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
1/1/2021
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - بكتريولوجيا
الفهرس
Only 14 pages are availabe for public view

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Abstract

• Tomonitortheabilityofthefood-borneopportunisticpathogenB.cereustosurviveduringminimalprocessingoffoodproducts,this studywasdesignedtodeterminetheisolationrateofB.cereusgroupbacteriaindifferentdairyproductsandfastfoodssamples andtostandupontheirphysiologicalandmolecularadaptive responsesagainstheat,acidicpH,coldas wellasosmoticsalt stresses.Inaddition,theE.coligrowthretrievalupon supplementationwithB.cereusorganicextracellularfractionswas alsoassessed.
• Atotalof200variousmilk,milkproductsandfastfoodsamples collectedfromdifferentsupermarketsandrestaurantswas subjectedfordeterminingtheexistenceofB.cereusgroupspecies byconventionalmicrobiologicaltechniques.Theeffectsofheat, cold,acid,andsaltonB.cereusheatresistancewas physiologically evaluatedthroughdetectionoftheirsurvivalandthermotolerance, whiletheyweremolecularlyevaluatedthroughmeasuringthegene expressionofB.cereusheatinducedproteins(mainlychaperones; GroELandDnaJ) byRT-qPCR assay.Finally,theassessmentofE.coligrowthretrievaluponsupplementationwithB.cereusorganic extracellularfractionscomparingwithinorganicsupplementswas phenotypicallyandgenotypicallydone
• Ourdatashowedthat B.cereus-likebacteriawereisolatedfrom manycategoriesoffoodstuffs(dairyproductandfastfoods) samples,whileitisfrequentlypresentassporesinthe environments.Inonly7%themilkandfastfoodssamples,the countswere>104CFU/mLorg,whereasin4.5%ofthesamples,