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Abstract This study was conducted throughout the two successive seasons of 2017 and 2018 at Fruit Nursery of Horticulture Department, Faculty of Agriculture at Moshtohor, Benha University Qalyubeia Governorate, Egypt., to study the impact of NPK bio fertilization application on different growth parameters and nutritional status as well as genatic relationships between different pomegranate genotypes of Manfaloty and Wonderful pomegranate transplants at one-year- old. The eight treatments involved in this study were summarized as follows: T1-recommended doses (RD) , T2-RD NPK mineral fertilizers + Soil application of Nitrobene at 5 ml/ transplant, T3-RD NPK mineral fertilizers + Soil application of Nitrobene at 10 ml/ transplant, T4-RD NPK mineral fertilizers + Soil application |