الفهرس 
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Abstract
This study examined the apoptotic and anti-oxidant effects of Artemisia annua extract together with its effect on levels of tumor markers as CA 15-3 and carcino-embryonic antigen. Effects of Artemisia annua extract on the mRNA expression of AKT1, BAX, CYP1A1, ERBB2, GST-P, MAP3K1, NKAP, p53, PCNA, PDK1, PIK3CA, PIK3R1, and VEGF were detected. Histopathological examination of breast tissue together with immunohistochemical examination of Bcl-2, calponin-h2, p63, and estrogen receptor were done. Our results showed significant decline of levels of total antioxidant capacity, SOD, CAT, GSH and GPx levels in the DMBA administered rats with significant elevation in Artemisia annua extract treated groups (P<0.05). Significant increase of MDA level in DMBA administered rats was detected with significant decline in Artemisia annua extract treated groups (P<0.05). Significant up-regulation of mRNA expressions of AKT1, CYP1A1, ERBB2, GST-P, MAP3K1 and PIK3ca were detected in DMBA administered rats with significant normalization in Artemisia annua extract treated groups (P<0.05). We observed the up-regula-tion of NKAP, PCNA, and PDK1 genes in DMBA administered rats, which were significantly normalized in the treated groups, particularly with 100 and 200 mg/kg of A. annua extract. Mammary gland of DMBA administered adult female rat showed invasive carcinoma with solid variant of invasive carcinoma. CA15-3 and CEA levels were significantly elevated in DMBA administered group in comparison to control, Artemisia annua extract 100 and 200 mg/kg of body weight. Treatment with Artemisia annua extract 100 and 200 mg/kg of body weight significantly ameliorated CA 15-3 and CEA levels. Finally, based on the biochemical, genetic, histopathological and immunohistochemical results we recommend the use of Artemisia annua extract as a promising complemen-tary drug in case of breast cancer.