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العنوان
Detection of class 1 integron in antibiotic resistant Campylobacter species
isolated from different sources/
المؤلف
Ali, Mona Mohammed Hamdy Mohammed.
هيئة الاعداد
باحث / مني محمد حمدي محمد على
مشرف / أحمد محمد عمار
مشرف / نورهان خيري عبدالعزيز
مناقش / علاءالدين حسين مصطفى
مناقش / احلام عبدالعزيز غريب
الموضوع
Bacteriology. Campylobacter.
تاريخ النشر
2021.
عدد الصفحات
209 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2021
مكان الإجازة
جامعة الزقازيق - كلية الطب البيطرى - بكتــريولوجيـــــا
الفهرس
Only 14 pages are availabe for public view

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Abstract

The genus Campylobacter is of great importance to public health. Poultry is a major reservoir for Campylobacter species and the broiler gut, especially the caeca is particularly colonized by C. jejuni.
The present study was carried to spot the light on the genus Campylobacter as a foodborne microorganism with great importance to public health and antimicrobial resistant genes, which may be transferred from food products of chicken and animal origin to human. Thus, it could be achieved through microbiological identification of the selected food pathogen, phenotypic characterization of antimicrobial resistance patterns of the recovered isolates, molecular characterization of class1 integrons among PDR, XDR and MDR isolates and investigation of the integron gene cassettes by sequencing of PCR products to correlate resistance phenotypes with the presence of integrons resistance genes.
Five hundred and fifty samples were collected from fresh and canned chicken, meat and milk from different supermarkets as well as human stool samples from different private laboratories in Zagazig City, Sharkia Governorate to be examined bacteriologically for the detection of campylobacters and identification of thermophilic campylobacters to the species level. Campylobacter species were detected in 58.11% of analysed chicken samples represented as 67.53% C. jejuni and 32.47% C. coli. C. jejuni were reported in 51.42, 74.28 and 66.67% of examined minced meat, raw milk and human stool samples, respectively.