الفهرس 
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Abstract
This study was conducted on beef burger, luncheon and sausage (35 of each) collected from different supermarkets at Menoufia Governorates for Detection of virulence genes in isolated E.coli and S.aureus using Multiplex PCR of E. coli and S. aureus. The data obtained from this study showed that the incidence of serologically identified E. coli was O1, O26, O125 and O142. Multiplex PCR showed Shiga toxin 2 gene (stx2) detected in O26 and O125, while shiga toxin 1 gene (stx1) detected in O1, O142 and O26. While O26 Positive E. coli strains for stx1, stx2, eaeA and hylA genes, O1, O142 Positive E. coli strains for stx1, eaeA and hylA and O125 Positive E. coli strains for stx2 and hylA. Regarding to S. aureus was isolated from examined products showed that 7 strains were toxin-producing and the most detected enterotoxin gene in the examined strains was SEA (2 isolates), followed by SEC (3 isolates), then SEB and SEA (1 isolates) and SED and SEA (1 isolates) and 8 strains were negative for 4 enterotoxin-producing genes.