الفهرس 
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Abstract
Objective: To assess the efficacy of agitation of chlorhexidine (CHX) and Silver nanoparticles AgNpss with 810nm diode laser or sonic endoactivator compared to side –vented needle on infected root canals with Enterococcus E Faecalis biofilms. Material and Methods: one hundred and ten extracted human premolars with a single oval canal were instrumented by protaper system up to F3. Biofilms of E. faecalis were generated based on a previously established protocol. Two teeth were used to check the biofilm formation, then the remaining Teeth were randomly divided into three equal experimental groups according to activation techniques used: group 1 (810 nm diode laser with 1 watt), group 2 (sonic endoactivator) and group 3 (Side vented needle). Each group was further divided into three equal subgroups according to the irrigant solution into; subgroup A: chlorhexidine, subgroup B: silver nanoparticles and subgroup C: distilled water: colony forming units (CFUs) and Confocal laser scanning microscopy CLSM were used to assess bacterial effect and viability. Data were analyzed by appropriate statistical analyses with P = 0.05. Results: Regarding the activation method, all groups had a significantly high percentage of dead bacteria (P<0.05). However, Laser was the highest and Endoactivator the least. Diode laser agitation of AgNpss irrigant showed the highest reduction percentage of bacteria (78.1%) with no significant difference with both Endoactivator and side vented needle using CLSM. Conclusion: Under the condition of the present study; results reinforced that laser activation (810 nm diode laser agitation of AgNpss or Chlorhexidine) was more effective in disinfection of oval root canals than endoactivator and side vented needle techniques, but without a significant difference. Keywords: Dentinal infection Silver-nanoparticles, CFUs, CLSM, Diode Laser, Sonic endoactivator.