الفهرس | Only 14 pages are availabe for public view |
Abstract Bioremediation of hydrocarbon pollutants in the environment is natural and continuous biological processes to clean the nature. Nature is enriched by wide array of bio-decomposers that are potential agents for degrading petroleum hydrocarbon. Biodegradation processes depend on both microbes and pollutants. Environmental degradation and frequent oil spill are compelling reasons for us to explore and search for local hydrocarbon degrading microorganisms from Egyptian soil in Ismailia by selective enrichment technique. In this attempt, 15 strains of microbes had been isolated that are able to use hydrocarbon compounds as a sol carbon source. These strains were cultivated in liquid media supplemented with n-alkanes and the combination of other mineral nutrients (e.g. nitrogen, phosphorus, magnesium, and sulfur) in the inorganic form. Among these, 14 strains are belonging to fungi that are capable of degrading hydrocarbons. All the fungal isolated strains are belong to five genera fusarium, Aspergillus, Gliocladium, Acremonium, and Pencellium.Only one strain was found to belong to the genus Streptomyces. Indeed, this actinomycetes strain showed a powerful degrading capability compared to the eukaryotic fungal strains. Biotransformation of hydrocarbons to biomass was observed by dry weight increase that is conclusive indicator of microbial utilizations and degradations of oil in biometric flasks. Fungal strains were unable to produce bio-surfactant, however, Streptomyces isolate was able to produce significant amount of bio-surfactant. Growths of microbes on hydrocarbon were strongly correlated with bio-surfactant production. Indeed, bio-surfactant production is a prerequisite for microorganisms to handle hydrophobic hydrocarbons. These studies help investigate new local strains for hydrocarbon degradation that are attractive candidate for bioremediation of petroleum hydrocarbon. |