الفهرس | Only 14 pages are availabe for public view |
Abstract A total of 120 random samples of chicken products represented by chicken breast, thigh, liver and gizzard (25 of each) were collected from different markets in Menofia governorate, Egypt. The results revealed that the incidence of Campylobacter spp. was positive 49/120samples. The highest incidence was found in chicken liver (56.67%), gizzard (53.33 %) followed by thigh (30 %) and breast (23,33%). It is evident from results that the incidence of C. jejuni, C.coli and C.lari were 6.67 %, 10% and 6.67 % in breast ;C. jejuni , C.coli and C.lari and C.cinaedi were 13 %, 6.67 %, 6.67% and 3.33 % in thigh; C. jejuni, C.coli, C.lari and C.cinaedi were 16.67 %, 20 %, 3.33 % and 13.33 % in gizzard and C. jejuni; C.coli ,C.lari, C.cinaedi and C.upsaliens were 23.33%, 16.67 %, 10 %,6.67% and 3.33% in liver, respectively. Latex Agglutination test is confirmatory identification of C.jejuni in examined samples ,it percent was 15%for 18 samples. Validity of examined samples of chicken meat and giblet(120samples) depending on their contamination with C.jejuni was102/85% accepted whereas unaccepted samples was19/15.83% . The antimicrobial susceptibility of isolated C.jejuni were examined and the results revealed that C.jejuni showed resistance against to streptomycin, erythromycin, nalidixic acid, amikacin, neomycin, cefotaxim, sulphamethoxazol, ciprofloxacin, chloramphenicol, oxytetracycline, kanamycin, cephalothin, ampicillin and gentamicin were 100%,94.5%, 77.8%, 66.7%,61.1%61.1%, 50%, 44.4%, 44.4%33.3%, 27.8%,22.2%,11.1% and 5.5% individually. Furthermore, PCR used as confirmatory tool for detection Campylobacter spp. in positive samples(18/100%) by 23SrRNA (650bp). The occurrence of virulence genes of C. jejuni strains isolated from all the examined samples of chicken carcasses. Virulence genes cdtA, cdtB and cdt C, cdtA and cdtB, cdtA and cdtC, cdtB and cdtC were present in 44.4%, 11.1%, 16.7% and 27.8% of examined of strain of C.jejuni respectively. The public health importance of the Campylobacter spp. and methods for protecting consumers were also discussed. |