الفهرس 
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Blood components & their indicationsOnly 14 pages are availabe for public view
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Abstract
In transfusion medicine, it is an important issue to optimize the ex vivo storage conditions for platelet rich plasma (PRP) to preserve platelet integrity and to minimize storage lesions (Cauwenberghs et al., 2007).Although, platelet transfusion can save patient’s life, it bears potential risks like other blood products, so that in some cases transfusion reactions are more common in platelet transfusion than red blood cell transfusion (Spiess et al., 2004). These reactions occur in a wide range, from an allergic reaction (the most common one) or mild fever to acute hemolytic reacts, Transfusion-Related Acute Lung Injury (TRALI), anaphylaxis or sepsis (Tormey et al., 2009).the outcomes of platelet transfusions are not always easy to predict in terms of bleeding control or increase in platelet count. Reasons could be disease-specific factors, fever or infections leading to platelet consumption, but the complement system may also be involved (Meinke et al., 2019).The blood complement system has a wide array of functions associated with a broad spectrum of host defense mechanisms, including both antimicrobial and antiviral actions. Complement system is involved in allergic reactions and is activated by alternative, classic and lectin pathways and plays a role in effective immune surveillance. Products derived from the activation of complement components include important non self recognition molecules C3b, C4b, and C5b as well as the anaphylatoxins C3a, C4a, and C5a, which induce a variety of cellular immune responses. These anaphylatoxins can also act as potent proinflammatory agents (Ricklin et al., 2010).Given the importance of injection efficacy of platelet products in prophylaxis and treatment of patients as well as evaluation of potential effects of complement system activation on patient and quality of product, this study investigated the effect of preparation and storage methods of platelets on complement activation level in these products In the current study, Platelet Rich Plasma can be stored for 5 days under standard storage conditions without affecting the activity of the complement system (C3a and C4d biomarkers assays).mean C3a level was compared among days separately and revealed non statistically significant difference thanks to presence of alternative pathway inhibitors that prevent its activation As regard C4d, elevation of C4d level among days had no significant difference due to absence of triggering factors that activate classical pathway.Storing PRP for 5 days resulted in significant reduction of platelet count. This was most observed on fifth day of storage compared to other days. The reason for this is accumulation of platelets metabolites that enhance their apoptosis. Therefore, PRP can be used before the fourth day of storage to avoid marked reduction of platelet count.No statistically significant difference was detected regarding culture results among 5 days of PRP storage.