الفهرس 
Introductionيوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
The aim of the work was to study the differential expression of long non coding RNA in hepatocellular carcinoma, on top of chronic HBV and HCV infections.
This study was conducted on 182 persons included 45 patients with hepatocellular carcinoma on top of chronic HCV, 40 patients with hepatocellular carcinoma on top of chronic HBV, 50 patients with liver cirrhosis and 47 healthy persons of matched age and sex as controls. Patients were 108 (80%) males and 27 (20%) females. Their ages were ranging between 35-75 years old. An informed consent was obtained before patients entered the study.
Patients and controls were classified in to the following groups: -
group A: Comprised 45 patients with hepatocellular carcinoma on top of chronic HCV
group B: Comprised 40 patients with hepatocellular carcinoma on top of chronic HBV
group C: Comprised 50 patients with liver cirrhosis.
group D: Comprised 47 healthy persons as a control group.
Diagnosis of liver cirrhosis and cancer was done by clinical examination, radiological evaluation and laboratory investigations.
For this purpose, all patients were subjected to the following: Full history taking, full clinical examination and investigations including: Complete blood count, liver and kidney function tests, erythrocyte sedimentation rate, serological tests for viral markers by ELISA (HCVAb and HBsAg), HCV RNA and HBV DNA by real time PCR,serum alpha-fetoprotein (AFP) level by ELISA and - Quantitative real time -PCR for lncRNA, ANRIL, aHIF and hPVT1 genes as a target, GAPDH gene as an endogenous reference gene, abdominal ultrasonography and triphasic CT.