الفهرس 
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Abstract
The objectives of this study were to determine the presence of some virulence and antibiotic resistant genes of Escherichia coli isolated from vaginal discharge samples from cattle with clinical endometritis. Also, to evaluate the performance of MALDI-TOF-MS analysis under real routine laboratory conditions.
Materials and Methods: A total of 138 vaginal discharge samples were collected from Holstein cows suffered from post-partum endometritis for bacteriological examination and serotyping identification. The antibiotic sensitivity test was carried out for E. coli strains by using VITEK 2 automated system. All different E. coli strains were chosen for genotyping characterization of some virulence and antibiotic resistant resistance genes by using PCR. Finally, MALDI-TOF-MS technique was applied for all variant E. coli strains to confirm its identification.
Results: A total of 93(67.4%) E. coli isolates were obtained by examination of 138 samples. E. coli serotypes which obtained were O126, O55, O26, O86a, O63, O119, O111, O15, O114 and O142. The results of antibiotic sensitivity test revealed that, Ampicillin / Sulbactam, Piperacillin / Tazobactam, Ceftazidime, Ceftriaxone, Cefepime, Meropenem, Ciprofloxacin, Levofloxacin and Nitrofurantoin were the most effective antibiotics against all E. coli strains (100%). On the other hand, E. coli isolates resisted the action of Ampicillin, Amoxicillin / clavulanic acid, Tetracycline and Streptomycin (100%). The genotyping characterization of E. coli resistance and virulence genes by using PCR proved that, all strains harbored phoA, fimH, blaTEM & aadA1. And only O55, O86a, O111, O114 & O142 harbored eaeA. On the other hand, kpsMII & ibeA genes didn’t detected within any strains. MALDI-TOF-MS technique confirmed the isolates of E. coli with interpretation high confidence and performed the dendrogram for all E. coli strains according to the similarities of peaks which observed within each E. coli spectrum.