الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 117 |  |  |
| | | from | 117 | | |
Abstract
This work aimed to isolate the pathogens causing arthritis in ducks and identify them using VITEK 2 system (one of the latest technologies in the laboratory field) and cPCR. In addition to antimicrobial susceptibility analysis to all isolates. Arthritis causes great economic losses to the poultry industry in a negative way through the inability of the bird to go to places of food and water, which leads to a lack of the food conversion rate, causes low weights, leads to infection with other diseases and the consumption of a lot of antibiotics.
In this study, 109 diseased ducks including 55 one day old ducklings and 54 ducks (5 days to one year of age) were collected from 30 flocks of pekin, muscovy, shrefan, and mullard ducks from different localities suffered from lameness and inflammation of the joints and foot pad and 54 embryonated duck eggs collected directly from hatcheries.
The isolation was done on 3 different media (MacConkey agar, blood agar, and mannitol salt agar), the shape, size and color of the colonies were recorded, Gram staining and biochemical tests were performed, then 20 isolates were identified using VITEK 2, PCR was performed for E.coli, Staphylococcus, Salmonella and Klebsiella.
Out of a total of 48 isolates were detected either by VITEK 2 system or by PCR, the results were 26 isolates E.coli, 9 isolates Staphylococci, 6 isolates Salmonella, 5 isolates Klebsiella, and 1 .