الفهرس | Only 14 pages are availabe for public view |
Abstract T1DM is one of the more dangerous diseases because it is an autoimmune disease results from full destruction of ß-cells of Langerhans in pancreas by autoimmunity of patient causes increasing of both B.S.L. and urine sugar level. Most patients were normal people with a good health suddenlyattacked and symptoms begins, patients children and young people, some of them were diagnosed at the age of 6 months, large numbers of T1DM are misdiagnosed as T2DM leading to sever symptoms which may lead to death. Our search studied how the role of (HLADQA1exon2 gene) in mutations leads to T1DM. Our gene is one of HLA genes related to HLA class ΙΙ located on chromosome 6 we aimed to know the gene affected by physiological parameters of patients. Samples were collected from voluntaries’ intravenous blood, and were have some clinical analysis to determine degree of physiological damage. At the same time genomic analysis were performed for samples by using PCR to specify gene sequence, amplifying it , and using gel electrophoresis to isolate the gene for DNA sequencing. By using the Bioinformatic analysis, we collected more information about the gene and its role in T1DM--- using GENBEE program to predict the transcripted RNA from the gene predicting translated protein. We find that translated protein have some mutations as a result of miss,substitution, and add some nucleotides based on using SNP which lead to change the expected translated protein in groups no. A1,A2, A3, and A4 results on T1DM . By using ONLINEGENEBEE SERVICES we can predict the secondary structure of RNA results from HLA-DQA1 exon2 gene indicates that (stema) and ( free energy) released from groups (A1,A2,and A3) were less than that released from the same normal gene documented in NCBI ref. so rete if protein production VII. SUMMERY 112 in these groups were little more than that in normal in NCBIref. , but it is not functional, while in group A4 free energy produced was more than that in normal indicated in NCBI ref. ,so rate of protein production was less than that indicated in NCBIref. and normal group. We can conclude results in thissearch in: Physiological parameters in our cases were normal because of regular administration of their medication (insulin) ,while molecular genomic analysis for HLA-DQA1 exon2 geneclarified that there were a mutation in all cases when compared to normal in NCBI ref. . Finally, we wanted to shed light on the necessary of using molecular biological analyses as a diagnostic tool to emphasis cases with T1DM |