الفهرس 
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Abstract
The present study was conducted to evaluate the immune-stimulating effect CpG-ODN on broiler chickens vaccinated against Newcastle disease. The motifs examined with different vaccination programmes using different inoculation routes. 270 (one day old) broilers chicks were divided into 6 groups.. Antibodies responses were monitored based on hemagglutination inhibition and ELISA assay for detection of IgG. Average body weight gain and feed conversion ratio were estimated to evaluate CpG-ODN growth promoting effect. All vaccinated groups which supplied with CpG-ODN recorded higher antibodies titre than only vaccinated group. group 4 which vaccinated and inoculated intra-muscular with CpG-ODN recorded higher antibodies titre than other vaccinated groups. Antibodies titer was similar in group five and six without significant difference and both were lower than group 4. ELISA assay confirmed the significance between the adjuvated groups at 21 and 35 days of age. There were no significant differences (p≥0.05) were observed between different vaccinated and adjuvated groups in either average body weight gain or in feed conversion rate starting from day 7 of age and up to the end of experiment. In conclusion, CpG-ODN proved its efficiency as adjuvant when co-inoculated with Newcastle disease vaccines via different administration routes.
from the previous results, the following can be deduced:
- Co-injection of motif CpG-ODN used in the study as an adjuvant with ND vaccine could induce protective immunity against virulent ND virus.
- Intramuscular route for delivering of CpG-ODN produced the highest effect on antibodies titers.
-Intraocular application of the CpG ODN did not trigger the same response, as when administered parenterally.
-Co-administration of ND vaccine plus CpG-ODN didn’t enhance the performance (body weight) of broiler chickens.