الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
In the present study, a soil actinomycete was isolated from an area near the river Nile shoreline, Egypt (Nikla Al-Enab, Eitay El-Barod, Al-Behira). The identification of this isolate as S. griseorubens MWR7 was performed using 16S rRNA sequencing and analysis. The sequence has been deposited in the GenBank with the accession number LC066679. Factors affecting the biogenesis of AgNPs were optimized by applying the Plackett-Burman factorial design. The maximum silver nanoparticles (AgNPs) biogenesis (2.76 OD at 400 nm was achieved for the trial No.9 that contained the following ingredients (g/L): Starch (20.0);< MgSO₄ (0.05); K₂HPO₄ (1.0); NaNO₃ (2.0); AgNO₃ (1.0) mmol/l; pH (7.0); incubated at temperature 30 °C for 72 (h). The biosynthesized nanoparticles were characterized using spectroscopic techniques. AgNPs showed the characteristic UV spectra at a wavelength range of 300 - 600 nm and a characteristic absorption peak was recorded at the wavelength of 400 nm. For AgNPs with absorbance height of ≈ 2.56 a.u. and peak width at half maximum absorbance (PWHM) ≈120 nm, which remained constant over a long period of time indicating its stability. FTIR spectra showed the functional group of the biomaterials capping the AgNPs. EDX confirmed the formation of the metallic silver nanoparticles, indicating the presence of proteinaceous cabbing. TEM micrograph showed spherical AgNPs in shape with an average diameter of 22 nm. The biosynthesized AgNPs showed high stability up-to two weeks. The conjugate [AgNPs/cellulosic fiber (C)] of Luffa aeygptiaca and [AgNPs/activated carbon (AC)] was applied for drinking water treatment, which resulted in fecal coliform bacteria removal with a success of 99.9 % as a water treatment application.