الفهرس 
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Abstract
Two hundred fish (100 Mugil cephalus, 100 Nile tilapia) collected randomly
from (Manzala Lake and Sea water, respectively).for Pseudomonas species
isolation. (70%) Mugil and (87%) Tilapia were positive for Pseudomonas
presence. Bacteriological examination of 535 Pseudomonas isolates;
Pseudomonas fluorescens (25.42%), Pseudomonas aeruginosa (32.52%),
Pseudomonas putida (15.89%), Pseudomonas cepacia (10.1%), Pseudomonas
stutzeri (6.91%), Pseudomonas anguilliseptica (4.11%), Pseudomonas
alcaligenes (3.55%) and Pseudomonas acidovorans (1.5%). prevalence of
Pseudomonas in Oreochromis niloticus was isolated from intestine ,muscle,
liver, kidneys and surface with the following percentage 26.7, 23.31, 20.55
,16.87 and 13.2% respectively. prevalence of Pseudomonas in Nile tilapia was
isolated from intestine, surface, kidney, liver and muscle with the following
percentage (32.05, 28.23, 22.01, 11.01 and 6.7 %respectively). All 32 isolates
were sensitive to Ciprofloxacin, Gentamycin and Chloramphenicol and
resistant to Penicillin, Amoxicillin and Ampicillin/sulbactam. PCR revealed
that 32 tested isolates were Pseudomonas aeruginosa. 5 virulence genes
detected of P. aeruginosa isolates (16SrDNA) ; outer membrane lipoprotein L
(oprL); exotoxin S gene (exoS); exotoxin A gene (toxA) ; flagellin C gene
(fliC) and biofilming gene (pelA). as well as the antibiotic-resistance genes:
blaTEM and blaCTX genes.