الفهرس 
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Abstract
Enterococcus faecalis, an opportunistic fish pathogen, has been recognized as a
causative pathogen of hemorrhagic septicaemic disease as well as its bad effect
on public health. The present study aimed to investigate the incidence of
Enterococcus faecalis in fish with the characters of resistance and virulence of
its. A total number of 200 clinically diseased Nile tilapia (Oreochromus
niloticus)(112) and red belly tilapia (Tilapia zilli) (88) were collected from
commercial fishermen from Temsah brakish lake in Ismailia Governorate from
March 2020 to October 2020. The isolation and identification of E. faecalis from
tilapia were performed as well as the antimicrobial sensitivity test and PCR. The
prevalence of E. faecalis in Oreochromus niloticus and Tilapia zilli were 21.6%
and 26.8%, respectively. The isolates were highly sensitive to
chloramphenicol(C30) and resistance to Nalidixic acid (NA30), Vancomycin
(VA30), streptomycin(S10), Erythromycin(E15) and Ampicillin (AMP10). PCR
was used for confirmation of the identified colonies at 310 bp for 16sRNA
sequence. Molecular detection of some virulence genes showed presence of
specific bands of enterococcus faecalis at 566 bp for EF3314 with percentage
87.5%, presence of specific bands for gelE and asa1 at 213 bp & 375 bp
respectively with percentage 100% and showed no specific bands for esp gene.
The obtained results proved that is an emerging pathogen for Tilapia species in
Ismailia governorate.