الفهرس 
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Abstract
Atotal 150 fish, 50 Oreochromis niloticus and 50 Tilapia zilli were collected randomly from El-Temsah Lake and 50 clarias
gariepinus from fish farms in Ismailia Governorate were showing signs of septicemia and others apparently healthy and
Moreove, 60 human samples from patients in Suez canal university Hospital in Ismailia Governorate and 23 water samples from
El-Temsah Lake in Ismailia Governorate were collected for isolation of P.aeruginosa.The bacteriological examination revealed
that, 33.4% of all investigated samples were positive for P. aeruginosa. Among different sources, the prevalence of P.
aeruginosa was 34.33%, 28.3% and 21.7% in examined fish, human and water samples, respectively. The most predominant
fish species for P. aeruginosa was Clarias gariepinus (42.5%), followed by Oreochromis niloticus (34.5%) then Tilapia zilli
(26%). In fish, the highest prevalence was recorded in liver (38.35%) followed by kidney (30.58%) then spleen (19.9%) and the
lowest was in gills (11.17%). In human, the high isolation rate was obtained from pus of infected wound and burns (45%),
followed by sputum (25%) then urine samples (15%). The results of antibiogram revealed that P. aeruginosa were highly
sensitive to colistin (100%). PCR assay of 15 representatives biochemically confirmed P. aeruginosa, isolates were confirmed
genetically based on amplification of 16S rDNA gene with a specific band at 956 base pair. Significant 4 virulence genes were
amplified in 15 examined P.aeurginosa isolates to confirm their pathogenicity. The distributions of virulence genes were as
following: oprL (100%), toxA (100%) and lasB (46.67%). However, exoS gene could not be detected in any of examined
P.aeurginosa isolates.The distributions of resistant genes were as following: tetA(A)(100%), both sul1 and blaTEM (93.33%),
while blaSHV was detected in (86.67%) of isolates. Sequencing of blaSHV gene of P.aeurginosa isolated from human was
applied with accession number MZ700496 at GeneBank, which was 99.5% identical to sequence of blaSHV-1 gene of K.
pneumonia. While Sequencing of blaSHV gene of P.aeurginosa isolated from fish with accession number (MZ700497) and that
isolated from water with accession number (MZ700498) both were 100% identical to the sequence of blaSHV-204 gene of K.
pneumonia at GeneBank. blaSHV gene derived from fish and water samples being more related to each other than to that
derived from human isolates.