الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Renal injury is a serious disease with many complications, mainly causing declining of renal function in patients. Sepsis-associated acute kidney injury (AKI) is more severe with a high rate of mortality. Celastrol is a natural compound with many pharmacological actions as strong anti-inflammatory and antioxidant effects. Celastrol effects as a treatment for LPS-induced AKI were not studied yet. The aim of our work was to study the potential therapeutic effect of Celastrol on Lipopolysaccharides (LPS) induced acute renal injury in a mice model. Results showed that Celastrol reduced the pathological injuries of kidney induced by LPS in mice by reducing histopathological changes. Celastrol lowered the levels of blood urea nitrogen and serum creatinine in LPS-treated mice in a significant way, restoring kidney function. In addition, Celastrol alleviated oxidative stress as indicated by restoring the concentrations of oxidative stress markers as reduced glutathione and malondialdehyde in kidney tissues. Notably, Celastrol inhibited the increase of tumor necrosis factor (TNF)-α and interleukin-6 (IL-6) productions in the group of LPS-treated mice. Celastrol reduced caspase-3 expression in kidney tissues, indicating diminished renal cells apoptosis. Toll-like receptor 4 (TLR4) and nuclear factor kappa B (NF-κB) expressions were inhibited upon treatment by Celastrol. In conclusion, Celastrol has potential to be used in sepsis-associated AKI, as least partly due to its anti-inflammatory effect and inhibition of TLR4/NF- κB signaling pathway.