الفهرس | Only 14 pages are availabe for public view |
Abstract Ulcerative colitis is a chronic gastrointestinal inflammatory disease caused by a dysregulated immune response against different environmental triggers in genetically predisposed individuals. The role of internal micro-environment is outstanding in this regard where UC patients have consistently been shown to have less diverse microbiome compared to healthy controls along with shift towards pathogens and away from healthy microbiota composition. Targeting the gut microbiome through fecal microbiota transplantation has been appealing option for treatment of patients with UC based on the previous positive experience of such therapy in C. difficile. However, lack of standardization of the process is a major hurdle before approval of FMT for UC. selection of a donor with a potential positive outcome is an everwanted goal in the pursuit to an optimum FMT process. This open-label randomized study was conducted on 20 patients (10 males, 10 females) with active UC who received FMT from 10 healthy unrelated donors via either colonic or nasogastric routes with an aim of identifying the ideal donor associated with the best response in respective recipients. Patients with a Mayo score 5 – 10 (median 8), aged 22 -63 years old (Mean ± SD 44 ± 10.50), were randomized to either colonic (n = 12, 60%) or nasogastric (n = 8, 40%) FMT after matching for age, sex & disease activity. Both patient groups received 8 doses of FMT over 8 weeks. Donors were recruited after passing the FMT donor health questionnaire and donor screening program. Donors were 8 males (80%) and 2 females (20%) aged 22- 48 years old (Mean 33.6 ± 9.57), BMI range 23.51 – 30.04 kg/m2 (Mean 24.76 ± 2.93). For the purpose of the study, donors were assessed for their usual dietary intake using the EPIC food frequency questionnaire in addition to assessment of their gut microbiota composition to identify the diet pattern and gut microbiota composition associated with response in UC patients. Diet data was evaluated using the EPIC FETA programme. Fecal samples from donors were stored at –80º c before DNA extraction, PCR, and sequencing by next generation sequencing methods. Microbiota raw data was submitted for analysis using the Qiime Package |