الفهرس 
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Abstract
The present study was performed to investigate the effect of subclinical and clinical hypophosphatemia on hematological, biochemical, oxidative status, skeletal muscle and appraise the interrelation between phosphorous deficiency and vitamin D.
The experiment was carried out on 40 adult female water buffaloes belonging to different localities in Sharkia governorate, Egypt. These animals were divided into 3 groups as follow:
group (1) was ten apparently clinically healthy female water buffaloes and kept as a normal control.
group (2) was fifteen buffaloes suffered from moderate signs of phosphorous deficiency. The signs included anorexia, emaciation, weakness, paleness mucous membranes and stiffness in gait. Phosphorous level displayed a significant decline.
group (3) was fifteen buffaloes suffered from severe signs of phosphorous deficiency. The signs included all the above signs in addition to red urine and the animal might be recumbent. Phosphorous level displayed a highly significant decrease.
Via jugular vein puncture, two blood samples were collected from each buffaloe. The first blood samples were taken into dipotassium salt of EDTA tube for hematological examination. The second samples were drawn in clean tubes without anticoagulant for serum separation to be used in biochemical assessment.