الفهرس | Only 14 pages are availabe for public view |
Abstract This study was designed to throw spot lights upon S.aureus isolated from raw cattle milk, biochemical and In-Vitro anti-microbial sensitivity for it with special reference to some virulence genes, antibiotic resistance genes and MRSA strains.So, the present study was performed on a total of 300 milk samples from small scale producers and farms in Damietta governorate. The incidence of S.aureus in the examined samples in 33.3%.The results of antibiotic sensitivity tests for S.aureus revealed that there is resistance to penicillin, oxacillin, cefoxitin, norfloxacin and oxytetracycline antibiotics. PCR results revealed that presence of virulence genes (coa, hlg, tsst-1) in examined isolates. Also, the presence of antibiotic resistance genes in examined isolates (blaZ, tetK, norA) and detection of enterotoxins by PCR revealed the presence of Seb in tested isolates but no other enterotoxins were detected. Also, PCR detected mecA gene in MRSA strains and its relationship to Seb gene. |