Search In this Thesis
   Search In this Thesis  
العنوان
Advanced Bacteriological Studies On Toxigenic Strains of Staphylococcus aureus Isolated from Cattle /
المؤلف
Mohammed, Nehal Ezzat Youssef.
هيئة الاعداد
باحث / نهال عزت يوسف
مشرف / حمزة محمد ابراهيم عيد
مناقش / حلمي احمد تركي
مناقش / احمد احمد رفعت خفاجي
الموضوع
Staphylococcus. Cattle.
تاريخ النشر
2022.
عدد الصفحات
134 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
9/11/2021
مكان الإجازة
جامعة قناة السويس - كلية الطب البيطري - البكتريا والمناعة والفطريات
الفهرس
Only 14 pages are availabe for public view

from 153

from 153

Abstract

This study was designed to throw spot lights upon S.aureus isolated from
raw cattle milk, biochemical and In-Vitro anti-microbial sensitivity for it
with special reference to some virulence genes, antibiotic resistance genes
and MRSA strains.So, the present study was performed on a total of 300
milk samples from small scale producers and farms in Damietta governorate.
The incidence of S.aureus in the examined samples in 33.3%.The results of
antibiotic sensitivity tests for S.aureus revealed that there is resistance to
penicillin, oxacillin, cefoxitin, norfloxacin and oxytetracycline antibiotics.
PCR results revealed that presence of virulence genes (coa, hlg, tsst-1) in
examined isolates. Also, the presence of antibiotic resistance genes in
examined isolates (blaZ, tetK, norA) and detection of enterotoxins by PCR
revealed the presence of Seb in tested isolates but no other enterotoxins were
detected. Also, PCR detected mecA gene in MRSA strains and its
relationship to Seb gene.