الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 232 |  |  |
| | | from | 232 | | |
Abstract
The purpose of this work was to synthesise the polymer ferrite and laser dyes will be selected and synthesized in film form for some biomedical application. The UV –Vis spectra of the films will be examined at different conditions of irradiation. The microstructure will be study by XRD Spectra. Different techniques will be used to study the electrical and optical properties of these nanocomposite films. This research was conducted at the Solid State Physics Laboratory - Department of Physics - Faculty of Science - Tanta University and Medical Research Institute-Alexandria University. The aim of the research was to synthesis nanocomposite films, study the electrical properties and indicate the effect of additives on the structural, electrical and thermal properties, as well as study the optical properties for use in some biomedical applications. Using infrared and ultraviolet spectra, a complete characterization of the nanometer films was made using a transmission and scanning microscope. In addition to study the absorption and emission spectra of these complexes through electromagnetic spectrum during a certain period of time. The search steps were as follows: - A comprehensive reference survey on the subject of the study. - Preparation of nanocomposite films from the dissolved polymer and different proportions of ferrite prepared in the nano range and previously prepared by one of the chemical methods, and different proportions of laser dyes are added in different proportions. The nanocomposite films are prepared by deposition method. - X-ray diffraction spectrometry to study the effect of ferrite and dyes on the polymer structure. - Measurement of infrared absorption spectra to study the molecular structure and the effect of ferrite and dyes on the molecular structure. - Measurement of the ultraviolet and visible spectra and determination of the refractive index and the optical gap energy. - Measuring the electrical conductivity of the films as well as the dielectric constant as a function of temperature and thermal properties using differential gravimetric analysis (TGA) and thermal differential pickling (DTA). - Studying the effect of laser fluorescence on the prepared compounds and effect of exposing these compounds to laser beams on their physical properties for use in some biomedical applications for the treatment of breast cancer (Ehrlich’s tumors implanted in experimental mice) by photodynamic therapy. The present work aims to cure Ehrlich ascites carcinoma tumor implanted to group of mice as an experimental animals using a photodynamic modality in combination with electromagnetic waves and polymer/ferrite-pomegranate nanocomposites as a photo sensitizer drug. Two sources of energy were used; namely laser and electromagnetic wasves. A total of 130 male Swiss albino mice with age 60– 65 day, weighing 20 25 g, were purchased. The animals were housed in plastic cages and were kept under natural light with diet and water at available. When the tumor had grown to about 10 mm in diameter at day 10 after inoculation, the treatment study was started. Use of experimental animals in the study protocol was carried out in accordance with the ethical guidelines of the Medical Research Institute, Alexandria University (Guiding Principles for Biomedical Research Involving Animals, 2011). Mice were grouped into Normal mice without treatment, tumor bearing mice without treatment and treated groups Results of the study: • The injection of the photosensitizer (polymer/ ferrite-pomegranate nanocomposites only without activation) has little effect on the tumor volume. • The effect of exposing the tumor to laser as a photodynamic therapy increased with increasing the laser energy resulted in decreasing the tumor volume, tumor growth rate and increasing tumor volume inhibition ratio. These effects were observed either on using laser alone or in the presence of the polymer/ ferrite-pomegranate nanocomposites. • According to laser results the inhibition in the tumor volume has maximum value on using laser in the presence of the polymer/ ferrite-pomegranate nanocomposites. • According to electromagnetic results the inhibition in the tumor volume has maximum value on using EM in the presence of the polymer/ ferritepomegranate nanocomposites. • Combined treatment of laser and EM waves in the presence of polymer/ ferrite-pomegranate nanocomposites was more effective than either laser or EM alone. • In the group of mice carrying the tumor only, a significant increase in the levels of MDA as compared to the control group of animals. The combination of photodynamic and EM therapy in the presences of polymer/ ferrite-pomegranate nanocomposites decreased significantly the levels of MDA. • In all Ehrlich bearing mice groups decreased activities of enzymatic antioxidants (SOD, CAT, GR, GST and TAC) in comparison with normal group were observed. • A significant increase in the enzymatic and non-enzymatic antioxidant guard was observed in the groups subjected to combination of photodynamic and EM therapy in the presences of polymer/ ferrite-pomegranate nanocomposites. • It was observed that treatment with polymer/ ferrite-pomegranate nanocomposites ameliorated the levels of serum creatinine and urea which is an indication of renal protection. This also confirms the protective role of polymer/ ferrite-pomegranate nanocomposites against renal toxicity. Also treatment with polymer/ ferrite-pomegranate nanocomposites protected against increase in serum levels of ALT, AST, and GGT, which is an indication of hepatoprotection by polymer/ ferrite-pomegranate nanocomposites. This also confirms the protective role of polymer/ ferritepomegranate nanocomposites against hepatotoxicity. • The histological evaluation revealed that all tumors from the group of mice bearing the tumor included highly malignant cells. Tumors excised from animals receiving treatment protoporphyrin showed significant areas of necrosis compared to groups without sensitizer. In the group of animals subjected to combination of photodynamic and EM therapy in the presences of polymer/ ferrite-pomegranate nanocomposites, large foci distinct necrosis areas were appeared.