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Abstract This research was performed to evaluate the potential role of Omega- 3 on the effect of energy drinks on the testis of adult male albino rats by using different histological and immunohistochemical techniques. This study was carried out on 40 adult male albino rats weighing from 180 to 200 grams. They were divided into four main groups: • Control group (I): It included 10 rats that received no treatment for the same periods as the experimental animals. They were used to study the normal histological structure of the testis. • Omega-3 group (II): It included 10 rats that received 300 mg/kg body weight of omega-3 orally using a gastric tube once daily for 3 months. • Energy drink group (III) : included 10 rats that received 1.07 ml/100 gm body weight of energy drink orally using a gastric tube once daily for 3 months. • Energy drink and omega-3 group (IV): included 10 rats that concomitantly received both omega-3 and energy drink at the same dose, route and duration as in groups II & III respectively. At the day after the last dose, all animals were anaesthized, sacrificed and their testes were taken to be subjected for different stains ( Haematoxylin and Eosin, Periodic Acid Schiff(PAS) |