الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Psoriasis is a common chronic autoimmune and inflammatory skin disease in which both genetic and environmental factors have a critical role. It affects 2- 3% of the world’s population.
Clinical manifestations of psoriasis are heterogeneous, ranges from limited to extensive disease. The majority of patients approximately (80%) have limited disease while approximately (20%) have extensive disease.
The most characteristic lesions consist of red, scaly sharply demarcated, erythematous papules and plaques present particularly over extensor surfaces and the scalp. The disease is variable in extent, duration and periodicity of flares.
The etiology of psoriasis is unknown, but evolving evidence suggests that it is a complex disorder caused by interaction of multiple factors includes immunological, environmental and genetic factors. It has been recognized as a systemic disorders associated with various comorbidities.
Psoriasis is characterized by hyperproliferation of the epidermis, antigen presentation, cytokine production by T-helper1, and angiogenesis.
Cold-inducible RNA-binding protein (CIRP), also called CIRBP or A18 hnRNP, it is a member of the cold shock protein family. It is a glycine-rich RNA chaperone that facilitates RNA translation.
Cold-inducible RNA-binding protein (CIRP) has been defined as an inflammatory mediator and a damage-associated molecular pattern (DAMP), which is highly expressed during trauma and shock.
Summary
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It can induce a variety of cellular responses including the release of proinflammatory cytokines and endothelial dysfunction.
Recent studies reported that CIRP binds to the TLR4/MD2 complex on both CD8+ and CD4+ T-cells to induce their activation and stimulates the release of TNF-α and HMGB1 from antigen-presenting cells.
This study aimed to evaluate the expression of CIRP, (serum and tissue), in psoriasis patients and to correlate this expression to the clinico-pathological data of the patients.
This case-control study included twenty patients presented with psoriasis. They were selected from Outpatient Clinic, Dermatology, Andrology and STDs Department, Faculty of Medicine, Menoufia University and twenty age and sex matched healthy subjects as a control group who attending Plastic Surgery OutpatientClinic, Menoufia University.
Exclusion Criteria included dermatological diseases other than psoriasis, history of malignancy, inflammatory diseases or symptoms of any infections.
After obtaining a written consent all patients were subjected to complete history taking, dermatological examination and registration of PASI score. 3 millileters of venous blood samples were taken under complete aseptic conditions from every participant.The samples centrifuged for 10 min at 4000 r.p.m.. The serum obtained for assessment of serum CIRP by ELISA technique. Skin biopsies were taken under local anaesthesia from all cases (lesions) and controls for histopathological and immunohistochemical evaluation using H&E and CIRP antibody stains.
Summary
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In the current study, Positive expression of Cold inducible RNA protein (CIRP) was observed in all cases (20 cases 100%) in lesional and prelesional tissue in addition to control group (20 cases 100%) with nuclear localization in all of the stained cells.
A significant difference in CIRP epidermal expression between lesional, perilesional cases and controls was observed. It was higher in control epidermis than perilesional skin and the lowest in lesional skin.
There was a highly significant difference between cases and controls regarding mean serum cold inducible RNA binding protein level, as it was higher in cases than controls (P value <0.001)
There was a significant positive correlation between CIRP serum level and H score of immunohistochemical expression in lesional epidermis (P value = 0.007)
Psoriasis patient with nail affection showed lower H score value of CIRP expression in lesional epidermis (mean=112, P value = 0.009).
Psoriasis patients presented with itching showed higher percentage of CIRP immunohistochmical expression in lesional epidermis (mean=97.5, P value = 0.047).
Psoriasis patients exhibiting dilated blood vessels in papillary dermis showed lower percentage of CIRP expression in lesional epidermis (mean = 86.7, P value = 0.046).
Serum cold inducible RNA binding protein was significantly lower in cases with dilated blood vessels in dermis (mean=208.6, P value = 0.023)
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There was no significant relation between H score of lesional epidermis and histo-pathological data of the studied cases was found.
There was no significant relation between serum cold inducible RNA binding protein and clinical data of the studied cases was found
So we recommend Further large scaled studies to validate our results.