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Abstract A total of one hundred random samples of fresh chilled and frozen beef livers (50 samples of each) were collected from different abattoirs and supermarkets at Cairo and Giza governorates to be examined for detection of antibiotic groups residues, hormonal (testosterone, estradiol, zeranol and trenbolone) residues and ractopamine residues. Twenty percent of fresh chilled samples were positives for antibiotic residues while not detected in frozen samples using FPT; however 40% of fresh chilled samples and 4% of frozen samples were positives for antibiotic residues using microbiological bioassay. Hormonal and ractopamine residue levels were below MRLs using ELISA. Cooking completely eliminate oxytetracycline residues but reduce sulfadimidine residues by 7%. Freezing at -20o C has no effect on sulfadimidine and reduce oxytetracycline residues by 8.7% after 3 months. Public health importance of such residues were discussed. |