الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
 |  | from | 85 |  |  |
| | | from | 85 | | |
Abstract
Dengue fever (DF) is an important mosquito-borne viral zoonosis affecting over 100 countries worldwide and exposing about 3.9 billion people to risk of infection. The disease has re-emerged in Egypt however, there is a paucity of recent epidemiological data available.
Therefore, this study aimed to: determine the prevalence and the associated risk factors of DENV in camels and humans in two southern Egyptian Governorates namely, Asyut and Sohag by using Camel Dengue Virus IgG(DV-IgG)ELISA Kit and Human Dengue Virus IgG(DV-IgG)ELISA Kit .
A sum of 91 dromedary camels including (37) imported and (54) native camels of 6 to 10 years old and including (76) males and (15) females were enrolled in this study, on examination of camels 65 of them apparently healthy and 26 of them had fever.
Our results revealed that:
1- The overall prevalence of dengue virus among 91 camel samples recorded by Enzyme-linked immunosorbent assay for detection of Dengue virus IgG (DV-IgG) was 3.3%.
2- Samples of camels were collected from Sohag Governorate were seropositive to DENV infection with 3.8% while, camel samples collected from Asyut Governorate were seronegative.
3- Concerning the effect of gender on DENV infection in camels results,revealed that DENV infection was higher (6.67%) in females in comparable to males (2.63%) and the difference was statistically non-significant.
4- Prevalence of DENV infection in camels was nearly similar in younger age (6-9 years )(3.45%) and older age (10-13 years) (3.23%).
5- Camel breed did not significantly affect the disease prevalence; however, imported camels were seropositive to DENV infection with 5.4% and imported camels were at 3 times greater risk compared to local breed camels with a percentage of 1.85%.
6- Our results illustrates that breeding purpose did not affect significantly the seroprevalence of DENV infection in camels and camels reared for work purpose were infected with DENV in higher percentage (4.17%) than camels reared for slaughtering purpose ( 2.33%).
7- All the camels with fever had antibodies against DENV (11.54%) however, camels without fever were seronegative .
A total of 91 human were included in this study represented by patients admitted to Asyut and Sohag Fever Hospital (66) were suffering from pyrexia and highly break bone fever and camel handlers (25) without any symptoms. Questionnaire was designed for each patient to determine the risk factors assessments regarding residence , age, gender, contact with camel and presence of break bone fever.
Our results revealed that:
1- The overall prevalence of dengue virus among 91 human samples detected by Enzyme-linked immunosorbent assay for detection of Dengue virus IgG (DV-IgG) was 12.1% .
2- DENV infection in humans was nearly similar in Sohag Governorate (12.12%) and Asyut Governorate (12%).
3- In this study rural residents had higher exposure to DENV with (13.04%) than urban residents of humans (9.09%).
4- Males had a higher seroprevalence (14.04%) than females (8.82%).
5- The age group ranging from 1 to 20 years was at the highest risk of getting the infection compared to the other two groups 21-40 years and 40 years and above.
6- Contact with camels did not affect the risk of DENV infection in human and the prevalence in camels handlers (12%) was nearly
similar with people who have no contact with camels (12.12%).