الفهرس 
DECLARATIONOnly 14 pages are availabe for public view
 |  | from | 265 |  |  |
| | | from | 265 | | |
Abstract
Aim: The aim of this study was to compare the in vivo regenerative potential of bone marrow mesenchymal stem cells (BMSCs) and platelet-rich plasma (PRP) on tongue of streptozotocin induced diabetic rats.Methodology: Forty male albino rats weighing 250±25 g were randomly divided into four equal groups: 1- group I (control group): served as control. 2- group II (diabetic group): rats received a single intra-peritoneal injection of streptozotocin (60 mg/kg) for diabetes mellitus induction. 3- Group
III (diabetic + PRP treated group): as group II + local injection of PRP in rats tongue after one month of diabetes induction. 4- group IV (diabetic + BMSCs treated group): as group II + local injection of
BMSCs in the rats tongue after one month of diabetes induction. After 30 days, all animals were euthanized and all tongues were dissected. The right half of the anterior 2/3 together with the posterior 1/3 of the tongue were processed and examined by histologically and histomorphometrically. While
the left half of the anterior 2/3 was examined by scanning electron microscope. Real time PCR was used to detect the expression of proliferative gene Ki-67.
Results: The tongue of group II showed significant atrophic and degenerative changes as compared to group I. group III and group IV tongue showed marked histological improvements, being mo
noticeable in group IV than group III. The morphometric analysis performed for each group revealed a significant increase in the mean epithelial thickness and area percentage occupied by acini in group
III and group IV in comparison to group II (P < 0.05). However group IV (diabetic + BMSCs treated group) showed a significant increase in both outcomes relative to group III (diabetic + PRP treated
group) (P < 0.05). Moreover, the mean Ki-67 gene expression significantly increased in group IV (diabetic + BMSCs treated group) in comparison to group III (diabetic + PRP treated group) (P < 0.05).
Conclusions: The present study concluded that diabetes mellitus had degenerative effects on rats tongue. Both BMSCs and PRP were capable of regeneration of these atrophic changes. Yet, the regenerative effect of BMSCs was more significant than PRP.