الفهرس 
The predictive role of ?III-Tubulin and MicroRNA-200c in advanced breast cancer patients receiving taxanes therapy
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Abstract
Background: Locally advanced breast cancer (LABC) is a common clinical problem, particularly in Egypt.The overall survival of LABC remained depressing.Therefore, finding a good predictor for the efficacy of treatment has an important significance. Class ÝIII tubulin is an isotype of tubulin that has been associated with malignant transformation and cancer development.The miRNAs are small non-coding RNAs that play an important role in gene regulation of many biological processes.Objective: To evaluate the role of ÝIII-tubulin and miRNA-200c as predictor markers for the efficacy of neoadjuvant taxanes therapy in advanced breast cancer patients. Methods: One hundred and forty three female breast patients: 22 patients with benign breast diseases and 121 breast cancer patients receiving neoadjuvant taxane therapy divided to: 94 responder patients and 27 non responder patients were enrolled in the present study and paraffin sections were collected. Patients were followed up from date of first diagnosis till end of the study.The expression level of ÝIII tubulin protein was immunohistochemically evaluated and miRNA 200c was evaluated using real time PCR. Results were correlated with response to neoadjuvant taxanes therapy. Results: The ÝIII tubulin protein was significantly higher in estrogen receptor (ER) negative and progesterone receptor (PR) negative breast cancer patients (P< 0.011, P< 0.053; respectively). It was also observed to be significantly up regulated in triple negative and HER2 enriched ( non luminals) cases and down regulated in luminals cases (p< 0.023).The miRNA 200 c was significantly down regulated in patients responding to taxanes as compared with non responders (P< 0.009)