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العنوان
Assessment of Ipomoea batatas (L.) Lam. viruses and their potential wild hosts in Egypt /
الناشر
Amira Abdrab Elnaby Mohamed Mazyad ,
المؤلف
Amira Abdrab Elnaby Mohamed Mazyad
هيئة الاعداد
باحث / Amira Abdrab Elnaby Mohamed Mazyad
مشرف / Mahasen H. Ismail
مشرف / Wafaa M. Amer
مشرف / Amal Aboul Elela A. Farghaly
مشرف / Ahmad Kamal Elattar
تاريخ النشر
2021
عدد الصفحات
220 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
العلوم البيئية (متفرقات)
تاريخ الإجازة
23/10/2021
مكان الإجازة
جامعة القاهرة - كلية العلوم - Botany (Microbiology)
الفهرس
Only 14 pages are availabe for public view

from 271

from 271

Abstract

Globally, sweet potato (Ipomoea batatas (L.) Lam., family Convolvulaceae), is an important vegetable crop, unluckily, the viral infection induces a significant yield loss. The current study aims to identify viruses infecting sweet potato in Egypt and highlights the role of the interfacing weeds as viruses reservoir. The study covered the incidence of the six viruses (SPFMV, SPVG, SPLV, SPMMV, SPCSV and SPLCV) in different governorate in Egypt and focused on the two dominant potyviruses sweet potato feathery mottle virus (SPFMV) and sweet potato virus G (SPVG) in details both of transmission, host range, inclusion bodies, virus morphology, histopathology, serological diagnosis, genome identification using RT-PCR and multiplex RT-PCR. The results revealed that the potyviruses SPFMV and SPVG are the most devastating viruses affecting sweet potato in Egypt. Coinfection with of both viruses was the common feature in both of sweet potato and its interfacing weeds. A wider host range in families Convolvulaceae, Chenopodiaceae and Euphorbiaceae, was detected. The infection symptoms on sweet potato were comparable to that detected in the interfaced weeds. The infection-incidence in perennial Convolvulaceae and Tiliaceae weed-species showed 100% virus incidence. The characteristic amorphous inclusion bodies for the detected viruses and the cytopathological alterations on the host cells were observed under Transmission Electron Microscopy (TEM).The nucleotide sequences of the partial coat protein gene of the detected viruses were compared to the isolate available in GenBank. Next generation sequencing analysis for the complete genome sequence of the two SPFMV and SPVG viruses