الفهرس 
Title : Control of some infectious diseases affecting cultured pangasius Spp. by recent bioproducts application
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Abstract
The present study aimed to identifying the main bacterial agent causing disease problem among P.hypophthalamus fish in semi intensive and intensive fish farms in Egypt and to assessment the efficacy of nucleotides (Nucleoforce{u2122}) on growth, hematological, non-specific immunological parameters and disease resistance in pangasius hypophthalmus fish. This study was conducted on 70 diseased Pangasius hypophthalmus fish collected from different Pangasius fish farms in Egypt. Bacteriological examination revealed the isolation of Pseudomonas aeruginosa that was biochemically confirmed using MALDI-TOF MS and VITEK 2 compact system. The prevalence and virulence of Pseudomonas aeruginosa were estimated genotypically using species specific 16S rDNA gene, OprL and ToxA virulent genes for detection of pathogenic strains followed by sequencing analysis. Pathogenicity of isolated Pseudomonas aeruginosa was confirmed by experimental intraperitoneal injection of healthy Pangasius hypophthalmus with positively virulent strains resulting in high mortalities among injected fish. Dietary nucleotides were incorporated in diet at different concentrations; group I control, group II 250 g/ ton diet and group III 500 g/ ton diet for 8 weeks. At the end of feeding trial, weight gain and specific growth rate (SGR) were recorded. Results showed that there was a significant increase in WG, SGR in fish groups received diet supplemented with nucleotides in comparison with the control group. Haematogram indices showed a significant elevation in RBCs count, HB value, WBCs and leukocytic count. Biochemically, High super oxide dismutase (SOD) activity, total protein and globulin levels were recorded in fish fed on 500g/t nucleotide (NT). Immunologically, Lymphocytic proliferation activity, Nitric oxide concentration and Serum lysozyme activity were continuously increased in 250g/t and the 500g/t NT treatment groups along feeding duration