الفهرس 
Relationship between biofilm formation and production of Extended-Spectrum ?-lactamases and Metallo-?-lactamases among Gram-negative bacilli
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Abstract
Gram-negative bacilli are a major cause of healthcare-associated diseases e.g. pneumonia, urinary tract infections and wound infections. However, the treatment of Gram-negative bacilli infections is getting more difficult because of emergence of increasingly resistant isolates to the available antibiotics. The ability of Gram-negative bacilli to form biofilm and produce Ý-lactamases (ESBL and MBL) could be the cause of their virulence and antibiotic resistance. The aim of this study was to detect biofilm, ESBL and MBL production among Gram-negative bacilli, to correlate and assess their antibiotic resistance pattern. Two hundred isolates of Gram-negative bacilli, were recovered from hospitalized patients at Cairo University Hospitals. They were collected from different clinical samples, subcultured on MacConkey{u2019}s agar and identified by the conventional microbiological methods. Antibiotic susceptibility testing was performed using Kirby-Bauer disc diffusion method. The ability of Gram-negative bacilli to produce biofilm was assessed using microtiter plate method. Gram-negative bacilli isolates were most frequently recovered from urine samples (53%). Biofilm formation was found in 47% of the isolates. Furthermore, the strong biofilm forming category was detected in 2% of the isolates, while the moderate and weak biofilm-forming categories were detected in 13% and 85% respectively. The production of ESBL and MBL among Gram-negative bacilli was 39.5% and 49% respectively. It is concluded that biofilm formation was associated with MBL production among Gram-negative isolates. In addition, ESBL and MBL producers were more resistant to antibiotics than non ESBL and non MBL producers