الفهرس 
In vitro evaluation of amebicidal effect of aloe vera extract and honey against acanthamoeba spp
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Abstract
This study investigated the in vitro effect of different concentrations of Aloe vera (A. vera) ethanol extract and honey against Acanthamoeba spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of Acanthamoeba infection) at different incubation periods (24, 48, 72 h). The tested concentrations were 100, 200, 400, and 600lg/ml for A. vera ethanol extract and 25, 50, 100, and 200lg/ml for honey. After each incubation period, the effect of A. vera extract and honey against Acanthamoeba cysts was assessed by counting the number of viable cysts, determining the inhibitory percentage and detecting the morphological alternations of treated cysts compared to non-treated and drug controls. A. vera ethanol extract and honey showed a concentration and time-dependent effect on the viability of Acanthamoeba cysts. In comparison with chlorhexidine, A. vera ethanol extract possessed a potent cysticidal activity at all tested concentrations throughout different incubation periods, except for concentration 100lg/ml which showed the lower inhibitory effect. With increasing the dose of A. vera ethanol extract to 200, 400, 600lg/ml, the recorded inhibitory percentages of Acanthamoeba cysts viability were 82.3%, 92.9% and 97.9% respectively, after 72 h compared to 76.3% of chlorhexidine. Similarly, honey at concentrations of 50{u2013}100lg/ml gave higher inhibitory effect of 59%and 76.7%, respectively compared to chlorhexidine which showed an inhibitory percentage of 55.7% after 24 h