الفهرس 
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Abstract
A prospective study was designed to last for six months from March
2021 to the end of August 2021. A total number of 126 chicken samples were
collected from five broiler chicken farms and two slaughter-houses located in
the Ismailia governorate. Each sample was composed of liver, intestine, and
breast and thigh muscles. The study revealed a total prevalence of 35.7% (45
positives out of 126 samples). Elkilo 7 and Abo-Swear slaughter-houses, AboSwear, Abo-Khalifa, Elmanaif, Sarabium, and Gabal Mariam farms revealed
prevalence up to 15.3, 23.0, 40.0, 35.0, 50.0, 45.0, and 30% respectively.
Initial isolation revealed a prevalence of up to 14.2% and delayed isolation
procedures revealed a prevalence of up to 21.42%. The bacteriological
analysis was carried out using traditional cultural means and molecular means
(PCR) targeting the invA gene. The isolated colonies in serological
identification revealed a majority of Salmonella Typhi O by 84% and
Salmonella Typhi H by 16%. The isolated Salmonella culture revealed higher
resistance incidence up to 100% against AMC (30 μg), AMP (10 μg), and
NAL (30 μg) followed by a resistance level up to 90% against ENR (5 μg),
and a resistance level up to 80% against DO (30 μg). The traditional culture
method revealed up to 83% sensitivity and 90% specificity while the
molecular analysis revealed up to 100% sensitivity and 100% specificity for
Salmonella detection in the admitted samples.