الفهرس 
Title : Investigating expression profiles for some circulatory microRNAs as potential biomarkers for the early diagnosis of HCV-associated liver disease progression in Egyptian patients
AbstractOnly 14 pages are availabe for public view
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Abstract
Hepatitis C virus (HCV) infection is characterized by persistent hepatic inflammation, leading to the development of fibrosis and cirrhosis. Hepatocellular carcinoma (HCC) incidence increases with HCV infection. Hence, early diagnosis is essential for prevention of HCV-associated liver disease progression. Circulatory microRNAs (miRNAs) have emerged as non-invasive and effective biomarkers for diagnosis of various diseases. This study aimed at investigating plasma miR-484, miR-524-5p, miR-615-5p and miR-628-3p expression signatures by RT-qPCR technique in Egyptian patients with HCV mediated fibrosis, cirrhosis and HCC cases as potential non-invasive biomarkers for early diagnosis and staging purposes. One hundred and twenty eight patients were recruited from Endemic Medicine Department, Kasr Al-Ainy Hospital at the Faculty of Medicine, Cairo University as follows; 47 HCV liver fibrosis sub categorized as 26 mild fibrosis and 21 advanced fibrosis, 40 HCV-cirrhosis and 41 HCV-HCC. Samples from forty gender- matched healthy volunteers were used in this study as healthy controls. Results showed that plasma miR-484 levels exhibited significant downregulation in advanced fibrosis group as compared to either mild fibrosis group or HCC group. Moreover, miR-484 showed significant upregulation in HCC group versus cirrhosis group. Both miR-524-5p and miR-615-5p were upregulated in cirrhotic group as compared to control group. Differential expression between HCC group and control group was noticeable in miR-524-5p. Receiver Operating characteristic (ROC) analysis revealed promising diagnostic performance for miR-484 in discriminating late fibrosis group from both mild fibrosis group and HCC group and also for miR-524-5p in distinguishing between cirrhosis group and fibrosis group. miR-524-5p was correlated with miR-628-3p in fibrotic group. In conclusion, miR-484, miR-524-5p, miR-628-3p could therefore serve as potential biomarkers for early diagnosis, prognosis and staging of various HCV mediated liver diseases