الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 120 |  |  |
| | | from | 120 | | |
Abstract
ABSTRACT
The present study was designed to investigated the chemical composition, identify the main constituents, the bioactive compounds and biological activities of Portulaca oleracea (purslane) (PO). Also, evaluated the effects of aqueous extracts of (PO) on human liver cancer (HepG2), human kidney cancer (Uo-31) cell lines and normal human lung cell line (Wi-38) as anticancer activity. from the obtained results the preliminary phytochemical screening of PO revealed the presence of flavonoids, phenolics, tannins, carbohydrate, steroids, saponine, amino acids and proteins. While, the chemical composition of purslane powder was characterized with high protein, ash and total carbohydrate, which amounted in 15.07±0.01, 28.29±0.05 and 40.82±0.60% on D.W, respectively.
The anticancer activity of extracts of Portulaca oleracea on two cancer cells line were exposed to various concentrations (0.4- 1.6- 6.3- 25- 100 μg/mL) for 24h. Percentages of cells viability were analyzed by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)), cellular morphology were evaluated by phase contrast inverted microscopy. from the obtained results showed that (PO) induced statistically significant (p<0.001) decrease in the cell viability of HepG2 cells in a concentration dependent manner. The HepG2 cells exposed to PO at 100 μg/mL and lower concentrations were found to be cytotoxic. The cells viability of (STA/HepG2) was recorded as 32.70, 42.94, 51.40, 60.44 and 66.67% at concentration 100, 25, 6.3, 1.6 and 0.4 μg/mL of (PO), respectively. The cell viability (S1/HepG2) was recorded