الفهرس 
Type 1 DiabetesOnly 14 pages are availabe for public view
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Abstract
ABSTRACT
Introduction: The influence of type 1 diabetes (T1D) on RBCs is multifactorial; shortened life-span, membrane deformability and altered lipid composition as well as enzymatic changes. All these changes possibly modify the oxygen-binding characteristics of hemoglobin (Hb). Objectives: Thus, the current study aimed to map the oxygen-binding characteristics of Hb among pediatric patients with T1D. Methods: A total of thirty-five patients with T1D and 35 matched healthy controls were enrolled. Raman spectroscopy ((l= 532 nm) was used to evaluate conformation of hematoporphyrin (HP) and Hb’s oxygen-binding properties. Specific Raman-scattering spectral lines were evaluated (maximum Intensities): I1355, I1375, I1550, I1580, I1172 cm-1. I1355/I1550 and I1375/I1580 reflect the relative ability of Hb to bind and release ligands respectively. (I1355/I1550)/(I1375/I1580) reflects affinity of Hb to ligands (Oxygen) and I1375/(I1355+I1375) reflects the relative amount of oxyHb (HbO2). I1375/I1172 reflects conformational changes of pyrrole rings.
Results: The intensities of I1355/I1550 and (I1355/I1550)/(I1375/I1580) were significantly lower among patients compared to healthy controls (p< 0.05). However, intensities of I1375/I1172 and I1375/(I1355+I1375) were significantly higher (p<0.05). Both I1355/I1550, and (I1355/I1550 )/(I1375/I1580) negatively correlated with duration of diabetes, HbA1c, cholesterol and urinary albumin execration (UAE) (P<0.05). Furthermore, the relative amount of HbO2 was found to be related to conformational changes in the pyrrole rings (I1375/I1172). Conclusions: In patients with T1D, Hb’s ability to bind ligands as well as its affinity to O2 was decreased. Conformational changes of HP directly influence oxygen-binding properties of Hb in T1D. The twostate allosteric model of Hb and Bohr’s effect could provide insights for oxygen-binding characteristics in T1D.