Search In this Thesis
   Search In this Thesis  
العنوان
Phenotypic and Genotypic characterization of Escherichia albertii in Chicken and Human diarrhea /
المؤلف
Abbas, Asmaa Abd- Elrahman
هيئة الاعداد
باحث / اسماء عبد الرحمن عباس محمد
مشرف / احسان عبد الصبور
مشرف / وجدان عبد الحميد
مناقش / خالد حسانين
الموضوع
Microbiology.
تاريخ النشر
2022.
عدد الصفحات
105 p.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
الناشر
تاريخ الإجازة
23/10/2022
مكان الإجازة
جامعة أسيوط - كلية الطب البيطري - ميكروبيولوجيا الطبية والمناعة
الفهرس
Only 14 pages are availabe for public view

from 129

from 129

Abstract

Escherichia albertii is a member of the Enterobacteriaceae family that gained a high interest. It is an emerging cause of foodborne infections leading to gastrointestinal infections in human. The organism isolated from animal and human.
To our knowledge, this study is the first that investigates the prevalence of E.albertii in human that suffered from gasteroenteritis and IBD, chicken and chicken products in Assiut City by conventional methods, including culture, biochemical and molecular identification techniques. Moreover, antibiotic suscepitibility of E. albertii to the various antibiotics was tested.
Chicken feces and products were randomly collected from different localities in Assiut City as sale points and poultry farms.
A total number of 225 human and chicken samples (75 human stool, 100 chicken feces and 50 chicken products) were collected. Human stool were collected from patients suffered from gasteroenteritis and IBD, they came to the outpatient clinics of the Tropical Medicine and Gastroenterology Department at Al Rajhi Liver University Hospital .
The prevelance of E.albertii was 28 % in chicken feces by culture, 26 % by biochemical tests and 20 % by PCR. No E. albertii could be detected in chicken products.
The prevelance of E.albertii in human that suffered from gasteroenteritis was 30% by culture, 22 % by biochemical tests and 8% by PCR. The prevelance of E.albertii in human that suffered from IBD was 24% by culture, 20% by biochemical tests and 20% by PCR.
Finally, the sensitivity of E.albertii was tested to 10 antibiotics including ampicillin (10μg), chloramphenicol (30μg), ciprofloxacin (5μg), Kanamycin (30μg), (streptomycin (10μg), sulfamethoxazole (25μg), gentamicin (10 μg), Cefixim (5μg). On the other hand, the resistance was observed for tetracycline (30 μg) and Nalidixic Acid (30 μg) with rate 65.5% and 51.7%, respectively.