الفهرس 
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Abstract
Summary
There is a great need for identificationof biomarkers and gene polymorphisms that could help in the diagnosis andunderstanding the pathogenesis of both rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) being two of the most commonchronic, disabling autoimmune diseases nowadays. We undertook this study to determinewhether circulating levels of interleukin-17 (IL-17) can serve as a useful biomarker in RA and SLEand to explore the potential associations between IL-17 inserum and the inflammatory markers (ESR, CRP) in both diseases.Moreover, we studiedthe effect of IL-17A ‐197G/A gene polymorphism and its different alleles on both diseases’ pathogenesis.
Samples anddata were obtained from thecase controlstudy of 90 subjects from the outpatient clinic of the Department of Internal Medicine, Immunology unit and the Department of Rheumatology and Rehabilitation, Beni-Suef University Hospital. Concentrations ofIL-17 levels weremeasuredby ELISA. Cases of RA and SLE had significant higher levels of IL-17 levels than controls (P-value<0.001), which confirms the contribution of IL-17 inboth diseases’pathogenesis. It also demonstrates its potential usage as an inflammatory biomarker to help in the diagnosis of the disease.
By using ROC curve, it was shown that the IL-17 marker could significantly predict the presence of SLE and RA. IL-17 can significantly predict the presence of RA at a cut-offof 9.3 with 80% sensitivity and 63.3% specificity, and SLE at a cut-off value of 10with 80% sensitivity and 73.3% specificity.
Interleukin-17serum levels was significantly positively correlated withESR and CRP levels which indicate that IL-17 probably participates in the mediation of inflammatory reaction and pathogenesis of both diseases.
As regarding IL-17A gene polymorphism, ‐197G/A SNP was identifiedusing polymerase chain reaction (PCR) based restriction fragment length polymorphism(RFLP) analysis.The frequencies of its different alleles showed no significant differences between RA and SLE cases and controls (P>0.05). This suggests that the IL‐17A (rs2275913) SNP may not beassociated with the susceptibility to these two diseases.